منابع مشابه
A Convenient Method for Solubilization and Refolding Recombinant Proteins: An Experience from Recombinant Mouse TGF-β1
Background: The production of recombinant proteins in Escherichia coli is one of the most valuable achievements in biotechnology, with many therapeutic and diagnostic applications; however, the aggregation and misfolding of proteins that result in the formation of insoluble inclusion bodies is a disruptive factor in this process. Various solubilization and refolding methods can be used to impro...
متن کاملSelecting appropriate hosts for recombinant proteins production: Review article
In recent years, the number of recombinant proteins used for therapeutic applications and industry has increased dramatically. Recombinant proteins are produced in many host organisms (microbial, insect, plant and mammalian cells). There are many factors to consider when choosing the optimal system for protein expression and purification including the mass, purity or solubility of the recombina...
متن کاملRefolding of recombinant proteins
Expression of recombinant proteins as inclusion bodies in bacteria is one of the most efficient ways to produce cloned proteins, as long as the inclusion body protein can be successfully refolded. Aggregation is the leading cause of decreased refolding yields. Developments during the past year have advanced our understanding of the mechanism of aggregation in in vitro protein folding. New addit...
متن کاملRecombinant proteins in plants
Background Over the last few decades, several studies have shown that plants can be a viable option for producing functional recombinant proteins with a wide range of structural characteristics [1]. In addition, the potential benefit for developing countries is a prominent feature that we have recently addressed [2]. Plant-produced recombinant proteins can already be considered a novel componen...
متن کاملDesign and Construction of Recombinant ELP-Intein Cassette for Use in Simple and new Purification Methods of Recombinant Proteins
Background and Objective: Use of elastin-like proteins (ELPs) provides high-performance protein purification without need for chromatography. In line with cost reduction and facilitation of recombinant proteins purification, which represent a high percentage of production costs, in this project, we eliminated the need for proteases in the process of separation of recombinant proteins from ELP b...
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ژورنال
عنوان ژورنال: Vox Sanguinis
سال: 2010
ISSN: 0042-9007
DOI: 10.1111/j.1423-0410.2010.01384.x